Ligninolytic enzymes of the white-rot fungus Phlebia radiata

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Ligninolytic enzymes of the white-rot fungus Phlebia radiata.

One oxidase (EC 1.10.3.2) and three lignin peroxidases (EC 1.11.1.-) were purified from the culture liquid of the white-rot fungus Phlebia radiata Fr. All the enzymes were glycoproteins. The oxidase had Mr 64,000 and the lignin peroxidases I, II and III had Mr values 42,000, 45,000 and 44,000 respectively. The lignin peroxidases were found to share common antigenic determinants: lignin peroxida...

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A beta-mannosidase was purified from Phlebia radiata grown in a medium containing wheat bran or galactomannan as a carbon source. Maximal activity was observed at pH 5.5 and at 50 degrees C. Highly purified isoforms of beta-mannosidase (GM-1, GM-2) isolated from media containing galactomanan and (OT-1) media with wheat bran were obtained by means of column chromatography on Q-Sepharose, SP-Seph...

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Ligninolytic enzymes of the basidiomycetous fungi Agaricus bisporus and Phlebia radiata on lignocellulose-containing media

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Production of ligninolytic enzymes by cultures of white rot fungi.

Some Basidiomycota were chosen for studies of key ligninases synthesis (25°C, 30 days) in modified medium (shaken or not cultures) with added wheat straw. Liquid Czapek medium with straw yielded a higher amount of laccase than peroxidase, ground straw induced enzyme worse than chopped straw. With peroxidase the reverse dependencies were observed. Laccase of Lentinus edodes synthesized two enzym...

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Sugarcane bagasse improves the activity of ligninolytic enzymes and decolourization of dyes by the white-rot fungus Pleurotus ostreatus

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1988

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj2540877